| Course Name | Molecular Biological Methods |
| Code | Semester | Theory (hour/week) | Application/Lab (hour/week) | Local Credits | ECTS |
|---|---|---|---|---|---|
| TLT 214 | Fall/Spring | 3 | 0 | 3 | 4 |
| Prerequisites | None | |||||
| Course Language | Turkish | |||||
| Course Type | Elective | |||||
| Course Level | Short Cycle | |||||
| Mode of Delivery | - | |||||
| Teaching Methods and Techniques of the Course | ||||||
| Course Coordinator | ||||||
| Course Lecturer(s) | ||||||
| Assistant(s) | - | |||||
| Course Objectives | Molecular biological methods are widely used for diagnosis and follow-up in medical laboratories. The aim of this course is to learn the working principles of molecular biological methods used in medical laboratories. |
| Learning Outcomes | The students who succeeded in this course;
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| Course Description | This course includes introduction to the general tools and materials used in molecular biology laboratory, working rules in molecular biology laboratories, isolation and amplification of DNA and RNA from blood and tissues, visualization and interpretation of amplified products in agarose gel electrophoresis, types of polymerase chain reaction, blotting, RFLP, strip test analysis, microarray and DNA sequencing methods. |
| Related Sustainable Development Goals | |
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| Core Courses | |
| Major Area Courses | X | |
| Supportive Courses | ||
| Media and Managment Skills Courses | ||
| Transferable Skill Courses |
| Week | Subjects | Required Materials |
| 1 | Introduction to the course, Introduction and tools used in Molecular Biology Laboratories | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 237-238. |
| 2 | Issues to be considered in Molecular Biology Laboratories and Contamination | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 242-246. |
| 3 | DNA isolation methods - 1 | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 239-240. |
| 4 | DNA isolation methods - 2 Spectrophotometric Determination of the Amount of DNA. | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 241-244. |
| 5 | Polymerase Chain Reaction (PCR) | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 250-258. |
| 6 | Preparation of Agarose Gel, Appliquing of PCR Products on Agarose Gel, Interpretation of Results, Acrylamide Gel Electrophoresis | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 250-258. |
| 7 | RNA Isolation and cDNA Handling, Reverse Transcriptase-PCR. | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 250-258. |
| 8 | Midterm Exam | |
| 9 | Multiplex and Real Time PCR | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 250-258. |
| 10 | Restriction Enzymes and Applications | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 265-269. |
| 11 | Microarray Technology and DNA Sequencing Analysis | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 299-305. |
| 12 | Blotting Methods (Nothern and Southern blots) | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 265-269. |
| 13 | Blotting Methods (western blot) | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 290-296. |
| 14 | Cell Culture | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 290-296. |
| 15 | General review | |
| 16 | Final exam |
| Course Notes/Textbooks | Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri. |
| Suggested Readings/Materials |
| Semester Activities | Number | Weigthing |
| Participation | 1 | 10 |
| Laboratory / Application | ||
| Field Work | ||
| Quizzes / Studio Critiques | 2 | 10 |
| Portfolio | ||
| Homework / Assignments | 3 | 15 |
| Presentation / Jury | ||
| Project | ||
| Seminar / Workshop | ||
| Oral Exam | ||
| Midterm | 1 | 25 |
| Final Exam | 1 | 40 |
| Total |
| Weighting of Semester Activities on the Final Grade | 60 | |
| Weighting of End-of-Semester Activities on the Final Grade | 40 | |
| Total |
| Semester Activities | Number | Duration (Hours) | Workload |
|---|---|---|---|
| Course Hours (Including exam week: 16 x total hours) | 16 | 3 | 48 |
| Laboratory / Application Hours (Including exam week: 16 x total hours) | 16 | ||
| Study Hours Out of Class | 15 | 2 | 30 |
| Field Work | |||
| Quizzes / Studio Critiques | 2 | 3 | |
| Portfolio | |||
| Homework / Assignments | 3 | 2 | |
| Presentation / Jury | |||
| Project | |||
| Seminar / Workshop | |||
| Oral Exam | |||
| Midterms | 1 | 10 | |
| Final Exams | 1 | 15 | |
| Total | 115 |
| # | Program Competencies/Outcomes | * Contribution Level | ||||
1 | 2 | 3 | 4 | 5 | ||
| 1 | To have the required contemporary theoretical and practical knowledge in his/her field | X | ||||
| 2 | To use the material and technology related to his/her field, and make their maintenance, use the information and communication technologies at basic level | X | ||||
| 3 | To have the competency to recognize the problems in his/her field, analyze them, develop evidence-based solutions and have the ability to share their suggestions with others | X | ||||
| 4 | To be aware of legal responsibilities, conduct basic studies in her/his field independently | X | ||||
| 5 | To communicate with patients, relatives and colleagues properly, comprehensively, honestly and explicitly, transfer his/her thoughts and knowledge through written and oral communication | X | ||||
| 6 | To take responsibility as an active team member during the practices in his/her field | X | ||||
| 7 | To commentate and evaluate the scientific information with a critical approach by the help of knowledge gained in his/her field | X | ||||
| 8 | To comprehend the importance of lifelong learning, to determine and meet her/his learning needs, to develop herself/himself by monitoring the development in science and technology | X | ||||
| 9 | To act by considering the universal ethical values, social and cultural characteristics | X | ||||
| 10 | To know the concepts of occupational safety, patient safety, environmental protection and quality, and fulfill the requirements | X | ||||
| 11 | To be able to follow information in his field and communicate with colleagues in English at least a level of European Language Portfolio A2 General Level | X | ||||
| 12 | To take necessary measures in accordance with laboratory safety and protection rules in the field of medical laboratory techniques | X | ||||
| 13 | To determine the needs according to the requirements of the laboratory processes and carry out activities for development | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest
